Thorn Media Recipe

From FreeBio

{Note: The following recipe makes 1L 1x Thorn media. This is the same as standard Yeast Complete Media, except with low-fluorescence yeast nitrogen base. This media is particular good for flourescent microscopy since the background autofluorescence is low. }

1. Fill a 2L glass bottle with 1L ddH₂O.
2. To the ddH₂O, add:
   • 5g (NH₄)₂SO₄
   • 1g KH₂PO₄
   • 0.5g MgSO₄
   • 0.1g NaCl
   • 0.1g Ca₂Cl
   • 0.5mg H₃BO₄
   • 0.04mg CuSO₄
   • 0.1mg KI
   • 0.2mg FeCl₃
   • 0.4mg MnSO₄
   • 0.2mg Na₂MoO₄
   • 0.4mg ZnSO₄
   • 2ug biotin
   • 0.4mg calcium pantothenate
   • 2mg inositol
   • 0.4mg niacin
   • 0.2mg PABA
   • 0.4mg pyridoxine HCl
   • 0.4mg thiamine
3. Autoclave to sterilize.